BBMRI
Proteomics / Academic service
NUPPA
Newcastle University Protein Production and Analysis
mass spectrometry-based, proteomics, data storage and analysis

Description

• Determination of the molecular weight of individual proteins using Ultra High Resolution Time of Flight Mass Spectrometry on a Bruker maXis 4G mass spectrometer

• Intact protein sequencing using T3 sequencing on MALDI-TOF

• Peptide mass fingerprinting

• Identification of proteins in single bands from SDS-PAGE gels

• Identification of proteins in solution or in biological fluids (such as plasma, urine, saliva)

• Determination of proteins that have changed in abundance when a biological system has been perturbed experimentally (quantitative expression proteomics) using techniques such as

o SILAC (Stable Isotope Labelling in Cell Culture)

o Isobaric labelling of peptides (using TMT, iTRAQ or IPTL)

o Label free quantitation using data-dependent acquisition or SWATH-MS

• Quantitation of drugs or their metabolites

• Discovery, verification or identification of proteins that have undergone post-translational modification (such as phosphorylation, ubiquitylation or glycosylation)

• Characterisation of protein-protein or protein-ligand interactions using analytical ultracentrifugation (AUC)

Provided resource

In addition to the experiments outlined in the 'technical aspects' section, we provide extensive consultancy for bespoke projects.

Contact information
Director, NUPPA Achim Treumann
Devonshire Building
Devonshire Terrace
Newcastle upon Tyne
NE1 7RU Tyne and Wear
United Kingdom
Telephone: +44 191 2084804